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Related Experiment Videos

Effect of opioids on Ca2+/cAMP responsive element binding protein.

W Bilecki1, R Przewłocki

  • 1Department of Molecular Neuropharmacology, Institute of Pharmacology, Polish Academy of Sciences, 12 Smetna St., 31-343 Cracow, Poland.

Acta Neurobiologiae Experimentalis
|February 24, 2001
PubMed
Summary

Calcium/cAMP response element binding protein (CREB) links opioid signaling to gene expression changes, influencing drug addiction behaviors. Opioid effects on CREB are biphasic, impacting gene induction after acute and chronic morphine administration.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Pharmacology

Background:

  • Calcium/cAMP response element binding protein (CREB) is a key transcription factor involved in secondary messenger pathways.
  • Opioid receptors interact with intracellular signaling cascades, including cAMP and calcium pathways.

Purpose of the Study:

  • To elucidate the role of CREB in mediating opioid effects on gene expression.
  • To understand how opioid-induced alterations in CREB impact drug addiction and dependence.

Main Methods:

  • Review of cellular and molecular mechanisms linking opioid signaling to CREB.
  • Analysis of CREB's role in gene expression changes in brain regions relevant to addiction.

Main Results:

  • Opioids regulate CREB levels, phosphorylation, and DNA binding, affecting genes linked to drug addiction.

Related Experiment Videos

  • CREB mediates opioid actions on gene expression in brain areas controlling drug-seeking behavior and dependence.
  • Altered CREB levels influence morphine's rewarding effects and cocaine self-administration.
  • Opioid signaling affects CREB through both cAMP-dependent inhibitory and calcium/PKC-dependent stimulatory pathways, explaining biphasic gene induction.

    • Conclusions:

    • CREB is a critical convergence point for opioid signaling pathways influencing gene expression.
    • The biphasic nature of opioid action on CREB contributes to the induction of CRE-controlled genes.
    • Other ATF/CREB family members and transcription factors modulate the duration of opioid-induced transcription.