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Synthesis and properties of 2'-deoxycytidine triphosphate carrying c-myc tag sequence
M Hinz1, D Gottschling, R Eritja
1Universität Ulm, Sektion Polymere, Germany.
Nucleosides, Nucleotides & Nucleic Acids
|February 24, 2001
Abstract:
The synthesis of 2'-deoxycytidine triphosphate carrying mercaptoethyl groups at position 4 of cytosine is described. This nucleoside triphosphate was reacted with a maleimido-peptide carrying the c-myc tag-sequence to yield a peptide-nucleoside triphosphate chimera. Primer extension studies showed that the nucleoside triphosphate modified with the peptide sequence is incorporated by DNA polymerases opposite guanine.