Related Experiment Video
Updated: Aug 16, 2026

Pyrosequencing for Microbial Identification and Characterization
Published on: August 22, 2013
Synthesis and incorporation of pyrrole carboxamide nucleoside triphosphates by DNA polymerases
1Medical Research Council, Laboratory of Molecular Biology, Cambridge, UK.
Abstract:
We have synthesised and examined the enzymatic incorporation properties of the 5'-triphosphates of 2'-deoxyribosyl pyrrole 3-monocarboxamide (dMTP) and 2'-deoxyribosyl pyrrole 3,4-dicarboxamide (dDTP). These analogues we had hoped would behave as ambivalent base analogues in that they can present two alternative hydrogen-bonding faces either by rotation about the carboxamide group or about the glycosidic bond. The two pyrrole derivatives, dMTP and dDTP, exhibit a preference for incorporation with Klenow polymerase. They are preferentially incorporated as either A or C.
Related Concept Videos
Proofreading
DNA Replication
Replication in Prokaryotes
DNA replication uses a large number of...
Translesion DNA Polymerases
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
Proofreading
Errors During Replication are Corrected by the DNA Polymerase Enzyme
Nucleic Acid Structure
DNA Structure
DNA has a double-helix structure. The...
Biosynthesis of Nucleic Acids

