Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Comparison among three methods for mycobacteria identification.

M Mondragón-Barreto1, C A Vázquez-Chacón, C Barrón-Rivero

  • 1Departamento de Biología Molecular y Departamento de Micobacterias, Instituto de Diagnóstico y Referencia Epidemiológicos, México, D.F., México.

Salud Publica De Mexico
|February 24, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical and bacteriological characteristics associated with clustering of multidrug-resistant tuberculosis.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2017
Same author

Drug resistance in Mexico: results from the National Survey on Drug-Resistant Tuberculosis.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2013
Same author

Multidrug-resistant pulmonary tuberculosis in Los Altos, Selva and Norte regions, Chiapas, Mexico.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2009
Same author

Evaluation of new external quality assessment guidelines involving random blinded rechecking of acid-fast bacilli smears in a pilot project setting in Mexico.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2005
Same author

[Drug resistance of Mycobacterium tuberculosis in Orizaba, Veracruz. Implications for the tuberculosis prevention and control program].

Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion·2001
Same author

Mycobacterium immunogenum sp. nov., a novel species related to Mycobacterium abscessus and associated with clinical disease, pseudo-outbreaks and contaminated metalworking fluids: an international cooperative study on mycobacterial taxonomy.

International journal of systematic and evolutionary microbiology·2001
Same journal

Glucose control among women with gestational diabetes and early offspring's growth.

Salud publica de Mexico·2026
Same journal

Economic impact of timely diagnosis and early referral in comprehensive cancer care in Mexico: the DERIVA strategy.

Salud publica de Mexico·2026
Same journal

A multi-dimensional index of rurality in Chile and its association with infant and neonatal mortality.

Salud publica de Mexico·2026
See all related articles

This study compared biochemical tests, HPLC, and PCR-RFLP for mycobacteria identification. A cost-benefit analysis proposed an algorithm prioritizing biochemical tests, followed by HPLC or PCR-RFLP for optimal identification.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Clinical Diagnostics

Background:

  • Accurate identification of mycobacteria is crucial for effective treatment and control of tuberculosis and other mycobacterial diseases.
  • Traditional identification methods can be time-consuming and may lack specificity for certain species.
  • Advancements in molecular techniques offer potential improvements in speed and accuracy.

Purpose of the Study:

  • To compare the efficacy and cost-effectiveness of biochemical tests, High-Performance Liquid Chromatography (HPLC), and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) for mycobacteria identification.
  • To develop an optimized diagnostic algorithm for mycobacteria identification based on a cost-benefit analysis.

Main Methods:

  • A total of 107 mycobacteria isolates were analyzed using biochemical tests, HPLC, and PCR-RFLP.

Related Experiment Videos

  • Comparative analysis of results against a reference laboratory standard was performed.
  • Statistical evaluation using the Q-Cochran test was employed to assess method concordance.

    • Main Results:

    • PCR-RFLP demonstrated the highest speed and specificity but incurred the highest cost.
    • Biochemical tests were effective for identifying Mycobacterium tuberculosis but were slow and costly for other mycobacteria.
    • HPLC offered a balance of price, speed, and specificity, positioning it as a mid-range option.

    Conclusions:

    • An identification algorithm is proposed, starting with biochemical tests.
    • If non-tuberculous mycobacteria are suspected, HPLC analysis is recommended.
    • PCR-RFLP should be used for ambiguous results, with DNA sequencing for novel patterns.