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Agrobacterium-mediated sorghum transformation.
1Trait and Technology Development, Pioneer Hi-Bred International Inc., Johnston, IA 50131, USA. zhaoz@phibred.com
Plant Molecular Biology
|February 24, 2001
Summary
This study successfully genetically transformed sorghum using Agrobacterium tumefaciens, achieving stable transformation events. Field-grown embryos significantly improved transformation efficiency, marking a breakthrough in sorghum genetic improvement.
Area of Science:
- Plant Biotechnology
- Genetics
- Agricultural Science
Background:
- Sorghum genetic transformation is crucial for crop improvement.
- Previous methods for sorghum transformation have limitations in efficiency.
Purpose of the Study:
- To establish and report a successful Agrobacterium-mediated genetic transformation protocol for sorghum.
- To optimize transformation parameters and assess factors influencing efficiency.
Main Methods:
- Agrobacterium tumefaciens-mediated transformation of immature embryos from public and commercial sorghum lines.
- Use of a 'Super-binary' vector with a bar gene for herbicide resistance selection.
- Optimization of transformation parameters and analysis of embryo source impact.
Main Results:
- Successfully generated 131 stably transformed sorghum events from 6175 embryos.
- Field-grown embryos yielded significantly higher transformation frequencies compared to greenhouse-grown embryos.
- Confirmed T-DNA integration via Southern blot and Mendelian segregation in T1 generation.
Conclusions:
- This is the first report of successful Agrobacterium-mediated stable transformation in sorghum.
- The developed method offers a higher transformation frequency than previously reported techniques.
- This advancement provides a valuable tool for genetic engineering and breeding of sorghum.