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Agrobacterium-mediated sorghum transformation.

Z Y Zhao1, T Cai, L Tagliani

  • 1Trait and Technology Development, Pioneer Hi-Bred International Inc., Johnston, IA 50131, USA. zhaoz@phibred.com

Plant Molecular Biology
|February 24, 2001
PubMed
Summary

This study successfully genetically transformed sorghum using Agrobacterium tumefaciens, achieving stable transformation events. Field-grown embryos significantly improved transformation efficiency, marking a breakthrough in sorghum genetic improvement.

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Area of Science:

  • Plant Biotechnology
  • Genetics
  • Agricultural Science

Background:

  • Sorghum genetic transformation is crucial for crop improvement.
  • Previous methods for sorghum transformation have limitations in efficiency.

Purpose of the Study:

  • To establish and report a successful Agrobacterium-mediated genetic transformation protocol for sorghum.
  • To optimize transformation parameters and assess factors influencing efficiency.

Main Methods:

  • Agrobacterium tumefaciens-mediated transformation of immature embryos from public and commercial sorghum lines.
  • Use of a 'Super-binary' vector with a bar gene for herbicide resistance selection.
  • Optimization of transformation parameters and analysis of embryo source impact.

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Main Results:

  • Successfully generated 131 stably transformed sorghum events from 6175 embryos.
  • Field-grown embryos yielded significantly higher transformation frequencies compared to greenhouse-grown embryos.
  • Confirmed T-DNA integration via Southern blot and Mendelian segregation in T1 generation.

Conclusions:

  • This is the first report of successful Agrobacterium-mediated stable transformation in sorghum.
  • The developed method offers a higher transformation frequency than previously reported techniques.
  • This advancement provides a valuable tool for genetic engineering and breeding of sorghum.