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Cytotoxic effect of methyl methacrylate on 4 cultured fibroblasts
M Taira1, H Nakao, T Matsumoto
1Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry, 1-3-27 Chuo-dori, Morioka 020-8505, Japan. mtaira@iwate-med.ac.jp
Purpose:
The objective of this study was to examine the cytotoxic effect of methyl methacrylate (MMA) monomer on 4 mammalian fibroblasts.
Materials And Methods:
Four cells--C3H10T1/2, L929, Balb/3T3 clone A31, and MC3T3-E1--were incubated for 6 days in 24-well microplates filled with 1 mL of Dulbecco's Modified Eagle medium containing MMA monomer that ranged from 0 to 200 mM/L. Cytotoxicity was evaluated by direct cell number count.
Results:
L929 possessed the largest TC50 value (the amount of MMA that caused 50% cell death) (34 mM/L), and Balb/3T3 clone A31 had the least value (1 mM/L); C3H10T1/2 and MC3T3-E1 had intermediate values (25 mM/L and 16 mM/L, respectively).
Conclusion:
This study showed that the MMA monomer had dose-dependent cytotoxicity, which varied among the cells tested.