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Subtyping of cytomegalovirus strains obtained from immunocompetent children

K Numazaki1, M Ikehata, S Chiba

  • 1Department of Pediatrics, Sapporo Medical University School of Medicine, Sapporo, 060-8543, Japan. numazaki@sapmed.ac.jp

In Vivo (Athens, Greece)
|February 24, 2001
PubMed

Insights

This study analyzed cytomegalovirus (CMV) genotypes in immunocompetent Japanese children, identifying diverse patterns in glycoprotein-B (gB), major IE (MIE), DNA polymerase, and glycoprotein-H (gH) genes. Restriction fragment length polymorphism (RFLP) proved valuable for CMV molecular epidemiology.

Area of Science:

  • Virology
  • Molecular Epidemiology
  • Genetics

Background:

  • Cytomegalovirus (CMV) is a common virus with diverse genetic profiles.
  • Understanding CMV genotypes is crucial for tracking its spread and impact, especially in specific populations.

Purpose of the Study:

  • To genetically characterize clinical isolates of cytomegalovirus (CMV) from immunocompetent Japanese children.
  • To evaluate the utility of restriction fragment length polymorphism (RFLP) in molecular epidemiological studies of CMV.

Main Methods:

  • DNA extraction from infected MRC-5 cells.
  • Polymerase chain reaction (PCR) amplification of CMV glycoprotein-B (gB), major IE (MIE), DNA polymerase, and glycoprotein-H (gH) genes.
  • Restriction fragment length polymorphism (RFLP) analysis for genotyping.

Main Results:

  • Analysis of 33 CMV clinical isolates revealed distinct genotype distributions for gB, MIE, DNA polymerase, and gH genes.
  • gB genotypes showed 24 group 1, 1 group 2, 6 group 3, and 2 mixed infections.
  • RFLP identified 18 MIE, 8 DNA polymerase, and 15 gH genotype patterns.

Conclusions:

  • CMV clinical isolates from immunocompetent Japanese children exhibit significant genetic diversity.
  • RFLP is an effective method for molecular epidemiological analysis of CMV infections in this population.

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