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Thin layer chromatographic identification of some sympathomimetic amines
Journal - Association of Official Analytical Chemists
|November 1, 1976
Summary
Thin layer chromatography effectively separated sympathomimetic amines using specific solvent systems. However, pharmaceutical amine mixtures were not resolved by the tested methods.
Area of Science:
- Analytical Chemistry
- Organic Chemistry
Background:
- Sympathomimetic amines are crucial in pharmacology.
- Chromatographic methods are essential for analyzing amine compounds.
Purpose of the Study:
- To investigate the thin layer chromatographic behavior of sympathomimetic amines.
- To evaluate different solvent systems and reagents for amine separation.
Main Methods:
- Thin layer chromatography (TLC) was performed on fiber sheets and glass plates.
- Sympathomimetic amines were analyzed using various acidic, neutral, and organic solvent systems.
- Reagents like bromocresol green and p-nitrobenzoyl chloride were employed.
Main Results:
- The benzene-ethyl acetate solvent system yielded two distinct spots for each amine standard.
- More polar amine spots demonstrated satisfactory separation.
- Pharmaceutical amine mixtures could not be resolved with any tested solvent system.
Conclusions:
- Specific solvent systems show promise for separating individual sympathomimetic amines.
- Further method development is required for analyzing complex pharmaceutical amine mixtures.