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Prostate cancer cell adhesion to quiescent endothelial cells is not mediated by beta-1 integrin subunit
C R Cooper1, L McLean, N R Mucci
1University of Michigan Comprehensive Cancer Center, Department of Internal Medicine, Division of Hematology/Oncology, Ann Arbor, Michigan 48109, USA. cacooper@umich.edu
Background:
Previous studies have reported that tumor cells' adhesion to quiescent endothelial cell is mediated by beta-1 integrins. The aim of this study was to determine the role beta-1 integrins play in prostate cancer cell adhesion to human bone marrow endothelial cells (HBME) and human aortic endothelial cells (HAEC).
Materials And Methods:
A well described blocking antibody to beta-1 integrin subunit was used in adhesion assays to determine the role of beta-1 integrin subunit in the adhesion of PC-3 cells to both HBME cells and HAEC.
Results:
Antibody to the beta-1 integrin subunit failed to reduce PC-3 adhesion to HBME and HAEC, yet this same antibody significantly reduced adhesion of PC-3 cells to fibronectin coated wells.
Conclusions:
The data suggest that metastasis of prostate cancer cells to bone may be mediated, in part, by preferential adhesion to HBME cells; but beta-1 integrins most likely are not involved in this interaction.