Related Experiment Video
Updated: Oct 9, 2026
Chromatin Accessibility Profiling in Whole Caenorhabditis elegans L4 Larvae
Published on: April 17, 2026
Application of reinforced frontal affinity chromatography and advanced processing procedure to the study of the
Y Arata1, J Hirabayashi, K I Kasai
1Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan. y-arata@pharm.teikyo-u.ac.jp
Abstract:
Frontal affinity chromatography is a very useful and simple method to analyze molecular interactions between an analyte and an immobilized ligand by calculating the extent of "retardation" of the elution front. We developed a very simple and efficient data-processing procedure that enables the measurement of very small differences in retardation with precision. This procedure was successfully applied to comparison of the binding properties of recombinant C. elegans galectins for their ligand.
