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Influence of colloid fluids on polymorphonuclear granulocyte function in vivo
1Department of Anaesthesiology and Intensive Care Medicine, Justus-Liebig-University, Giessen, Germany. Joerg.Engel@chiru.med.uni-giessen.de
Acta Anaesthesiologica Scandinavica
|February 24, 2001
Summary
Moderate infusions of colloids like human albumin (HA), gelatine (GEL), or hydroxyethylstarch (HES) do not affect granulocyte surface receptors in vivo. Further research is needed for high-dose effects in critical conditions like sepsis.
Area of Science:
- Immunology
- Critical Care Medicine
- Anesthesiology
Background:
- Granulocytes are key in immediate immune responses.
- Previous in vitro studies showed colloids increase complement receptors on neutrophils.
- This study aimed to verify these findings in vivo.
Purpose of the Study:
- To investigate the in vivo effects of common colloid solutions on granulocyte surface receptor expression.
- To determine the clinical significance of in vitro findings regarding complement receptors on neutrophils.
Main Methods:
- A randomized study of 60 orthopedic surgery patients (ASA-I).
- Patients received human albumin (HA), gelatine (GEL), hydroxyethylstarch (HES), or Ringer's solution.
- Flow cytometry analyzed granulocyte surface markers (CD11b, CD16, CD35, CD62L) pre-infusion and post-infusion.
Main Results:
- No significant changes in CR1 (CD35), CR3 (CD11b/CD18), Fc gamma receptor IIIb (CD16), or L-selectin (CD62L) expression on neutrophils.
- HA, GEL, HES, and Ringer's solution did not alter these key granulocyte surface receptors.
Conclusions:
- Moderate doses of HA, GEL, or HES do not acutely impact granulocyte adhesion or activation molecules in vivo.
- Potential effects of high-dose colloid administration in trauma or sepsis on neutrophil function remain speculative and warrant further investigation.