Apparatus for intracellular electrophysiological measurements at 200 ATA

An apparatus has been constructed and tested at pressures to 200 ATA which meets the basic requirements for intracellular microelectrode work. Standard microelectrodes, unaffected by pressure in this range, were used with lobster axon and frog sartorius fibers and action potentials have been recorded at pressure up to 151 ATA. The chamber itself has a simple roll-in door and a modular design that recommends it as a highly convenient multipurpose vessel for work at moderately high pressures.