Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immune complex processing in C1q-deficient mice.

J T Nash1, P R Taylor, M Botto

  • 1Rheumatology Section, Division of Medicine, Imperial College School of Medicine, London, UK.

Clinical and Experimental Immunology
|February 24, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Investigating the effectiveness of commercially available mouthwash on SARS-CoV-2 in vivo using viable virus titre as the primary outcome. A randomised controlled trial.

Access microbiology·2024
Same author

Executive Summary to the Royal Society report "COVID-19: examining the effectiveness of non-pharmaceutical interventions".

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2023
Same author

European survey on the current surveillance practices, management guidelines, treatment pathways and heterogeneity of testing of Clostridioides difficile, 2018-2019: results from The Combatting Bacterial Resistance in Europe CDI (COMBACTE-CDI).

The Journal of hospital infection·2022
Same author

Do probiotics prevent antibiotic-associated diarrhoea? Results of a multicentre randomized placebo-controlled trial.

The Journal of hospital infection·2020
Same author

Analysis of HLA-G expression in renal tissue in lupus nephritis: a pilot study.

Lupus·2019
Same author

A triglyceride-rich lipoprotein environment exacerbates renal injury in the accelerated nephrotoxic nephritis model.

Clinical and experimental immunology·2018
Same journal

The many faces of cytokine storm syndrome: immunopathogenic mechanisms and clinical implications for a better patient management.

Clinical and experimental immunology·2026
Same journal

PD-1 Engagement and Circulating Th17 Effector Activity During Secukinumab Treatment in Plaque Psoriasis.

Clinical and experimental immunology·2026
Same journal

Henoch-Schoenlein purpura (HSP) like lesions in IL12RB1 and IL12B defects - A multi-centric experience from India.

Clinical and experimental immunology·2026
Same journal

Case Series on the Efficacy of Daratumumab in Treating Patients with Anti-Interferon-Gamma Autoantibodies.

Clinical and experimental immunology·2026
Same journal

Deleterious germline CARD11 gain-of-function variants alter human B-cell and CD4+ T-cell differentiation and function.

Clinical and experimental immunology·2026
Same journal

Clinical, genetic, and functional characterization of novel NFKB1 variants in Chinese patients with primary immunodeficiency.

Clinical and experimental immunology·2026
See all related articles

Complement component C1q deficiency impairs immune complex (IC) processing in mice, reducing splenic uptake. This finding highlights the classical complement pathway's crucial role in managing immune complexes in vivo.

Area of Science:

  • Immunology
  • Complement System Biology
  • Autoimmune Disease Mechanisms

Background:

  • Immune complexes (IC) are processed by complement and Fcgamma receptors.
  • Defects in IC processing are linked to autoimmune diseases like lupus.
  • The role of complement component C1q in IC processing requires further elucidation.

Purpose of the Study:

  • To investigate the impact of C1q deficiency on immune complex processing in vivo.
  • To determine the specific organ sites and kinetics of IC clearance in C1q-deficient mice.

Main Methods:

  • Intravenous injection of radiolabeled Hepatitis B surface antigen/anti-HBsAg immune complexes (IC) into C1q-deficient and control mice.
  • Quantification of organ uptake (liver, spleen) at various time points to assess IC clearance.

Related Experiment Videos

  • Comparison of IC distribution and localization between C1q-deficient and control groups.

    • Main Results:

    • The liver and spleen were primary sites for IC uptake in both groups.
    • C1q-deficient mice showed significantly reduced splenic uptake of IC compared to controls.
    • An initial accelerated hepatic uptake of IC was observed in C1q-deficient mice, mirroring human hypocomplementaemia.
    • Later hepatic localization of IC was comparable between C1q-deficient and control mice.

    Conclusions:

    • The classical complement pathway, mediated by C1q, is essential for efficient splenic clearance of immune complexes.
    • C1q deficiency alters IC distribution, leading to increased initial hepatic uptake.
    • These findings in mice corroborate human studies on the importance of the classical complement pathway in immune complex homeostasis.