Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Urinary prostaglandins. Identification and origin.

J C Frölich, T W Wilson, B J Sweetman

    The Journal of Clinical Investigation
    |April 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Comment on: Do baseline characteristics predict response to treatment for low back pain? Secondary analysis of the UK BEAM dataset.

    Rheumatology (Oxford, England)·2007
    Same author

    Physiotherapy management of non-specific back and neck pain.

    Rheumatology (Oxford, England)·2006
    Same author

    Cyclooxygenase-2 inhibitors.

    Advances in protein chemistry·2001
    Same author

    Fibromyalgia... I mistake your shape.

    Rheumatology (Oxford, England)·2001
    Same author

    Sclerosing injections for chronic low back pain.

    Rheumatology (Oxford, England)·2000
    Same author

    Chronic fatigue syndrome in mother and child.

    Journal of the Royal Society of Medicine·2000
    Same journal

    Pharmacokinetics and pharmacodynamics of a long-acting monoclonal antibody against malaria in African adults.

    The Journal of clinical investigation·2026
    Same journal

    Extracellular matrix reprogramming by the YAP/TAZ- TGF-ß2 axis drives immune exclusion in cholangiocarcinoma models.

    The Journal of clinical investigation·2026
    Same journal

    Tumor cell-derived extracellular vesicles foster the immunosuppressive landscape of pancreatic cancer.

    The Journal of clinical investigation·2026
    Same journal

    Julie Zikherman receives the ASCI/Marian W. Ropes, MD, Award.

    The Journal of clinical investigation·2026
    Same journal

    Targeted degradation of MDM2 overcomes feedback regulation of p53 signaling in Merkel cell carcinoma models.

    The Journal of clinical investigation·2026
    Same journal

    SGLT2 inhibitors enhance ketogenesis by acting as allosteric activators of the mitochondrial enzyme HMGCS2.

    The Journal of clinical investigation·2026
    See all related articles

    Urinary prostaglandins, including Prostaglandin E2 and F2alpha, were detected in human urine using mass spectrometry. These findings suggest urine reflects kidney prostaglandin synthesis, offering a new tool for studying kidney function.

    Area of Science:

    • Biochemistry
    • Nephrology
    • Analytical Chemistry

    Background:

    • Prostaglandins are biologically active lipids with diverse physiological roles.
    • The presence and origin of prostaglandins in human urine have not been fully elucidated.
    • Understanding urinary prostaglandins is crucial for assessing renal function.

    Purpose of the Study:

    • To detect and quantify prostaglandins in human urine.
    • To investigate the renal origin of urinary prostaglandins.
    • To establish urinary prostaglandins as potential biomarkers for renal physiology and pathology.

    Main Methods:

    • Mass spectrometry, including selected ion monitoring, was employed for prostaglandin detection and quantification.
    • Derivatization techniques were used to enhance the detectability of Prostaglandin E2 and F2alpha.

    Related Experiment Videos

  • Prostaglandin synthesis was stimulated in vivo (dogs and humans) via arachidonic acid or angiotensin infusion to determine the origin of urinary prostaglandins.

    • Main Results:

    • Prostaglandin E2 and F2alpha were successfully detected and quantified in female human urine.
    • Infusion of arachidonic acid into a dog's renal artery increased urinary Prostaglandin E2 excretion.
    • Angiotensin infusion in dogs and humans led to increased urinary excretion of Prostaglandin E2 and F2alpha, indicating a renal origin.

    Conclusions:

    • Urinary prostaglandins, specifically Prostaglandin E2 and F2alpha, originate from the kidney.
    • Urinary prostaglandin levels serve as a reliable indicator of renal prostaglandin synthesis.
    • Urinary prostaglandins represent a valuable tool for investigating renal prostaglandin physiology and pathology.