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Accuracy of t-PA, u-PA, PAI-1 and PAI-2 estimation in human bile by ELISA kits
Z Kondera-Anasz1, A Michalski, D Gil
1Department of Immunology and Serology, Silesian Medical University, Katowice, Poland.
Unlabelled:
The aim of this study was to establish the accuracy of determination of fibrinolytic proteins in human bile using commercial kits.
Material And Method:
Gall bladder bile was obtained from patient undergoing laparoscopic cholecystectomy. The concentrations of tissue plasminogen activator (tPA), urokinase (uPA) and plasminogen activator inhibitor-1 (PAI-1) and inhibitor-2 (PAI-2) in bile were measured by enzyme linked immunoassay. Afterwards we conducted so called 'recovery test'.
Results:
Recovery percents of t-PA, u-PA, PAI-1, PAI 2 were very high i.e. 91.94, 94.07, 93.95, 91.2. We concluded that human fibrinolytic proteins in bile could be detected very accurately using commercial kits.