Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Nitric Oxide Signaling Pathway

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Physiological responses of Robinia pseudoacacia L. of different origins to soil nitrogen supply.

Plant biology (Stuttgart, Germany)·2026

Same author

Preclinical characterization and phase I results of TQB2102, a first-in-class HER2 biparatopic antibody-drug conjugate, in patients with advanced solid tumors.

Annals of oncology : official journal of the European Society for Medical Oncology·2026

Same author

[Efficacy of branch-first intervention with delayed aortic repair for acute type A aortic dissection with concurrent mesenteric malperfusion syndrome].

Zhonghua yi xue za zhi·2026

Same author

Dynamic stabilization and parametric excitation of instabilities in an ablation front by a temporally modulated laser pulse.

Physical review. E·2025

Same author

[Construction and application of a risk index of <i>Echinococcus</i> infection based on the classification of echinococcosis lesions].

Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control·2024

Same author

Mass online training of health care workers during COVID-19: approach, impact, and outcomes for over 10,000 health care providers.

Public health·2024

Search research articles

Related Experiment Video

Updated: Jul 25, 2026

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

Neuronal nitric oxide synthase: expression in rat parietal cells.

S Premaratne¹, C Xue, J M McCarty

¹Department of Medicine, Medical College of Virginia-Virginia Commonwealth University, Richmond, Virginia 23249, USA.

American Journal of Physiology. Gastrointestinal and Liver Physiology

|February 24, 2001

Summary

Nitric oxide synthases (NOS) are enzymes involved in gastric acid secretion. This study found neuronal NOS (nNOS) expressed in rat parietal cells, suggesting its role in regulating stomach acid production.

More Related Videos

Dissection of Pelvic Autonomic Ganglia and Associated Nerves in Male and Female Rats

Dissection of Pelvic Autonomic Ganglia and Associated Nerves in Male and Female Rats

Published on: March 7, 2020

Immunostaining-Based Detection of Dynamic Alterations in Red Blood Cell Proteins

Immunostaining-Based Detection of Dynamic Alterations in Red Blood Cell Proteins

Published on: March 17, 2023

Related Experiment Videos

Last Updated: Jul 25, 2026

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

Dissection of Pelvic Autonomic Ganglia and Associated Nerves in Male and Female Rats

Dissection of Pelvic Autonomic Ganglia and Associated Nerves in Male and Female Rats

Published on: March 7, 2020

Immunostaining-Based Detection of Dynamic Alterations in Red Blood Cell Proteins

Immunostaining-Based Detection of Dynamic Alterations in Red Blood Cell Proteins

Published on: March 17, 2023

Area of Science:

Physiology
Biochemistry
Molecular Biology

Background:

Nitric oxide synthases (NOS) produce nitric oxide (NO), a signaling molecule.
NO is implicated in regulating gastric acid secretion.
The specific NOS isoform in gastric oxyntic mucosa is not well-defined.

Purpose of the Study:

To determine the cellular localization of NOS in rat oxyntic mucosa.
To identify the specific NOS isoform expressed in parietal cells.
To investigate the role of NOS in gastric acid secretion regulation.

Main Methods:

NADPH-diaphorase histochemistry on rat oxyntic mucosa.
Immunohistochemistry using isoform-specific NOS antibodies.
Western blot analysis and RT-PCR on isolated rat parietal cells.

Main Results:

NADPH-diaphorase and nNOS immunoreactivity were localized to parietal cells in the oxyntic mucosa.
Isolated parietal cells demonstrated clear expression of nNOS via immunohistochemistry, Western blot, and RT-PCR.
Sequence analysis confirmed the amplified product as nNOS with 100% identity.

Conclusions:

Neuronal NOS (nNOS) is expressed in rat gastric parietal cells.
Endogenous NO produced by nNOS in parietal cells may act as an intracellular signal.
This suggests a role for nNOS in the regulation of gastric acid secretion.