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Pendrin: an apical Cl-/OH-/HCO3- exchanger in the kidney cortex
M Soleimani1, T Greeley, S Petrovic
1Department of Medicine, University of Cincinnati, Cincinnati, Ohio 45267-0585, USA manoocher.soleimani@uc.edu
American Journal of Physiology. Renal Physiology
|February 24, 2001
Summary
Pendrin (PDS) acts as a chloride/base exchanger in kidney cells. This protein is crucial for transporting chloride, hydroxide, bicarbonate, and formate in the proximal tubule and cortical collecting duct.
Area of Science:
- Nephrology
- Molecular Biology
- Cell Physiology
Background:
- The specific apical chloride/base exchangers in kidney proximal tubule and cortical collecting duct (CCD) cells are not fully identified.
- Pendrin (PDS), known for its role in the thyroid and Pendred's syndrome, functions as an anion exchanger.
Purpose of the Study:
- To investigate the distribution and function of pendrin (PDS) in the rat kidney.
- To determine if PDS acts as an apical Cl-/base exchanger in renal cells.
Main Methods:
- RT-PCR to assess pendrin mRNA distribution across kidney nephron segments.
- Immunoblot analysis to localize pendrin protein.
- Expression studies in HEK-293 cells to characterize pendrin's transport functions.
Main Results:
- Pendrin mRNA is abundant in the rat kidney cortex and enriched in proximal tubule suspensions.
- Pendrin protein is localized to cortical brush-border membranes.
- HEK-293 cells expressing pendrin demonstrated Cl-/OH-, Cl-/HCO3-, and Cl-/formate exchange activity.
Conclusions:
- Pendrin is identified as an apical Cl-/base exchanger in kidney proximal tubule and CCD cells.
- Pendrin mediates the exchange of chloride with hydroxide, bicarbonate, and formate in these renal segments.