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Hemoglobin E, an oxidatively unstable mutation.

H Frischer, J Bowman

The Journal of Laboratory and Clinical Medicine

|April 1, 1975

Summary

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A new test detected gross turbidity in blood samples, primarily linked to hemoglobin E, an unstable variant. This finding aids in identifying oxidative hemolysis susceptibility and differentiating hemoglobin E from other types.

Area of Science:

Hematology
Biochemistry
Genetics

Background:

Erythrocytic enzyme deficiencies can lead to various health issues.
Hemoglobin variants, such as hemoglobin E, are prevalent in certain populations.
Oxidative stress plays a role in red blood cell disorders.

Purpose of the Study:

To evaluate a new diagnostic test for erythrocytic enzyme deficiencies.
To investigate the association between observed turbidity and specific hemoglobin types.
To characterize the oxidative instability of hemoglobin E.

Main Methods:

A novel assay utilizing 2,6-dichlorophenolindophenol reduction by glutathione was employed.
Blood samples from 3,159 individuals across diverse geographical locations were analyzed.

Related Experiment Videos

Hemolysates were incubated with dichlorophenolindophenol to assess hemoglobin precipitation rates.

Main Results:

Gross turbidity was observed in 81 samples, predominantly from Southeast Asia.
78 of the turbid samples contained hemoglobin E (Hb EE or Hb AE).
Precipitation rates showed Hb EE > Hb AE > other hemoglobins, indicating Hb E's oxidative instability.

Conclusions:

The new test effectively detects abnormalities associated with hemoglobin E.
Hemoglobin E is an oxidatively unstable hemoglobin, increasing susceptibility to hemolysis.
This method allows for simple detection and differentiation of Hb E from Hb C and Hb O-Arabia.