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Secretion of hen egg white lysozyme from Kluyveromyces lactis
R Tanaka1, M Ishibashi, H Tokunaga
1Laboratory of Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University, Japan.
Bioscience, Biotechnology, and Biochemistry
|February 24, 2001
Abstract:
Hen egg white (HEW) lysozyme was correctly processed and efficiently secreted from an alternative yeast, Kluyveromyces lactis. We constructed secretion vectors using PHO5, PGK, and LAC4 promoters, and found that the highest secretion was obtained under the direction of the PGK promoter in non-selective rich medium. K. lactis secreted HEW lysozyme with two-fold higher efficiency than S. cerevisiae, estimated by using a K. lactis-S. cerevisiae shuttle vector.