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Supramolecular dynamics of gap junctions.

M L Rütz1, D F Hülser

  • 1Biologisches Institut, Abeteilung Biophysik, Universität Stuttgart, Germany.

European Journal of Cell Biology
|February 24, 2001
PubMed
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Newly formed gap junction plaques are crystalline and become pleomorphic as they age. This structural change correlates with channel inactivation and ubiquitylation, impacting cell communication.

Area of Science:

  • Cell biology
  • Membrane protein dynamics
  • Intercellular communication

Background:

  • Membrane proteins undergo structural changes during their life cycle.
  • Gap junction channels are crucial for cell-cell communication, formed by connexin hexamers (connexons).
  • Protein aggregation and supramolecular changes are observable in proteins like gap junctions.

Purpose of the Study:

  • To investigate the morphological changes of gap junction plaques during their life cycle.
  • To understand the relationship between gap junction plaque structure, ubiquitylation, and function.
  • To determine how inhibiting protein trafficking affects gap junction plaque formation and morphology.

Main Methods:

  • Inhibition of protein trafficking using brefeldin A (BFA).

Related Experiment Videos

  • Quantification of cell-cell coupling via calcein transfer and flow cytometry.
  • Examination of gap junction plaque degradation and formation using indirect immunofluorescence and immunogold labeling.
  • Assessment of plaque ubiquitylation and crystalline packing.

    • Main Results:

    • Newly formed gap junction plaques exhibit a crystalline morphology.
    • Plaque ubiquitylation is higher in non-crystalline, pleomorphic structures.
    • Inhibition and reversal of protein trafficking (BFA) altered plaque morphology and ubiquitylation.
    • Crystalline packing increased, and ubiquitylation decreased after BFA reversal.

    Conclusions:

    • Newly formed gap junction plaques are crystalline.
    • Plaque morphology transitions to a pleomorphic structure during aging.
    • This morphological dynamic correlates with gap junction channel inactivation and plaque ubiquitylation.
    • Changes in gap junction plaque structure impact cell-cell communication.