[Biochemical changes in thrombocyte concentrates stored for 5 days]

Area of Science:

• Transfusion Medicine
• Hematology
• Biochemistry

Context:

• Platelet concentrates are vital for treating bleeding disorders.
• Storage conditions significantly impact platelet viability and function.
• Buffy-coat derived platelet concentrates (PC-BC) require careful evaluation during storage.

Purpose:

• To assess biochemical and functional alterations in PC-BC stored at 20°C for up to five days.
• To monitor changes in lactate dehydrogenase (LDH), pH, pCO2, pO2, glucose, and lactate levels.
• To evaluate platelet aggregation and hypotonic shock response over the storage period.

Summary:

• Over five days of storage at 20°C, PC-BC showed increased LDH and lactate, decreased pH and glucose, and altered pCO2 and pO2.
• Platelet function tests revealed a decreased hypotonic shock response and slower aggregation with adenosine diphosphate (ADP).
• Despite these biochemical and functional changes, the study found no detrimental effect on the efficacy of stored PC-BC.

Impact:

• Provides crucial data on the stability and usability of buffy-coat derived platelet concentrates.
• Informs transfusion guidelines regarding the optimal storage duration and handling of platelets.
• Contributes to ensuring the safety and effectiveness of platelet transfusions in clinical practice.