Related Experiment Videos
Somatostatin blockade improves the proliferative response but not intestinal morphological growth after small bowel
T Castell1, I A Gómez de Segura, I Vázquez
1Department of Experimental Surgery, La Paz University Hospital, Madrid, Spain.
Objective:
To find out whether or not blockade of somatostatin improves intestinal adaptation after small bowel resection.
Design:
Laboratory experiment.
Setting:
Teaching hospital, Spain.
Subjects:
Eighty adult Wistar rats.
Interventions:
Animals underwent intestinal resection or sham operation (n = 40 each) and were treated with a somatostatin antagonist either intermittently or continuously in three different doses (n = 8 each).
Main Outcome Measures:
Bowel mucosal thickness, proliferation and concentrations of cAMP, somatostatin, insulin-like growth factor 1.
Results:
Intestinal resection induced a proliferative and morphometric increase of the mucosa; however, the antagonist increased proliferation only in those animals given the highest dose. Intermittent doses induced a proliferative effect that was stronger than that in the three continuous groups. There was no relationship between trophic stimulus and insulin-like growth factor 1 or cAMP, but somatostatin concentrations increased after the intermittent course.
Conclusions:
Somatostatin receptor blockade with an antagonist does not cause in normal rats an intestinal morphological adaptation process or increase it after resection; however, it did promote a proliferative stimulus in the crypts.