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Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Anchoring Junctions01:03

Anchoring Junctions

Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Boundary Conditions: Lossless Lines01:21

Boundary Conditions: Lossless Lines

Consider a single-phase, two-wire, lossless transmission line terminated by an impedance at the receiving end and a source with Thevenin voltage and impedance at the sending end. The line, with length, has a surge impedance and wave velocity determined by the line's inductance and capacitance.
At the receiving end, the boundary condition states that the voltage equals the product of the receiving-end impedance and current. This relationship is expressed as a function of the incident and...

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Related Experiment Video

Updated: Jun 29, 2026

Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

Scabrous complexes with Notch to mediate boundary formation.

P A Powell1, C Wesley, S Spencer

  • 1Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.

Nature
|February 24, 2001
PubMed
Summary
This summary is machine-generated.

Scabrous protein stabilizes the Notch receptor, sharpening boundaries of proneural clusters. This mechanism ensures the correct formation of single pioneer neurons during Drosophila retina development.

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Area of Science:

  • Developmental biology
  • Neuroscience
  • Cell biology

Background:

  • Pattern formation in epithelia is poorly understood.
  • Proneural clusters, marked by Atonal expression, are early pattern elements in the developing Drosophila retina.
  • Notch signaling is crucial for establishing and patterning Atonal expression.

Purpose of the Study:

  • To investigate the role of Scabrous in Notch signaling and proneural cluster formation.
  • To elucidate the molecular mechanisms underlying pattern sharpening in the Drosophila retina.

Main Methods:

  • In vivo studies in Drosophila melanogaster.
  • Biochemical assays to analyze protein interactions.
  • Analysis of Atonal expression and proneural cluster boundaries.

Main Results:

  • The secreted protein Scabrous associates with the Notch receptor.
  • Scabrous stabilizes Notch protein at the cell surface.
  • This interaction leads to sharpened proneural cluster boundaries.
  • Ensures the establishment of single pioneer neurons.

Conclusions:

  • Scabrous-mediated stabilization of Notch activity is essential for precise pattern formation in the developing retina.
  • The findings provide insight into how developmental processes establish sharp boundaries and single-cell fates.