DNA microsatellites linked to quantitative trait loci affecting antibody response and survival rate in meat-type...

Selection for immune response parameters may lead to improved general disease resistance. Because disease resistance and immune response are hard-to-measure quantitative traits with low to moderate heritability, they may respond more efficiently to marker-assisted selection (MAS) than to phenotypic selection. To detect DNA markers linked to quantitative trait loci (QTL) associated with immune response, a resource half-sib family of 160 backcross (BC1) and intercross (F2) birds was derived from a cross between two meat-type lines divergently selected for high or low antibody (Ab) response to Escherichia coli. By using 25 microsatellite DNA markers covering approximately 25% of the chicken genome, initial genotyping of 40% of the resource family was followed by complete genotyping of the entire family with four suggestive markers. Three of these markers exhibited significant association with immune response: (1) ADL0146 on Chromosome 2 associated with Ab to SRBC and Newcastle disease virus (NDV), (2) ADL0290 on linkage group 31 affecting Ab to NDV, and (3) ADL0298 on linkage group 34 associated with Ab to E. coli and survival. The family was also genotyped with five linked markers from two of the suggested regions, and interval mapping was applied. The results confirmed the significant effects, suggested the location of the QTL, and confirmed the genetic association between immune responses and disease resistance. These findings support the idea of improving poultry immunocompetence by MAS.