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Related Experiment Videos

HLA-G expression by tumors.

B Davies1, S Hiby, L Gardner

  • 1Department of Pathology, University of Cambridge, United Kingdom.

American Journal of Reproductive Immunology (New York, N.Y. : 1989)
|February 24, 2001
PubMed
Summary
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This study investigated HLA-G expression in tumors. Researchers found no evidence of HLA-G protein in tumor cells, suggesting it

Area of Science:

  • Immunology
  • Molecular Biology
  • Oncology

Background:

  • The non-classical MHC class I antigen, HLA-G, is expressed by trophoblast cells.
  • HLA-G is proposed to mediate immune evasion by the placenta from maternal uterine NK cells.
  • A similar mechanism is hypothesized for tumor cells escaping immunosurveillance.

Purpose of the Study:

  • To investigate the expression of HLA-G in various tumor types and cell lines.
  • To determine if HLA-G expression by tumor cells contributes to immune evasion.
  • To resolve conflicting data on HLA-G expression in tumors.

Main Methods:

  • Immunohistology on tumor tissue sections.
  • Flow cytometry and RT-PCR on tumor cell lines.
  • Analysis of NK cell infiltration in tumor deposits.

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Main Results:

  • No detectable HLA-G protein expression was found in any tumors or cell lines examined.
  • Very faint HLA-G mRNA bands were detected in three cell lines (hepG2, Mead, CaSki) by RT-PCR.
  • Tumor deposits were generally not infiltrated by NK cells.

Conclusions:

  • The study's findings do not support the hypothesis that tumor cells express HLA-G to evade NK cell-mediated lysis.
  • The proposed immune evasion mechanism involving HLA-G in tumors appears unlikely based on these results.