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Updated: Oct 9, 2026
Intestinal Epithelial Regeneration in Response to Ionizing Irradiation
Published on: July 27, 2022
A low dose of ionizing radiation increases luminal release of intestinal peptidases in rats
M Fittkau1, A Grothey, R Gerlach
1Institute of Clinical Chemistry and Pathobiochemistry, Martin Luther University Halle-Wittenberg, Halle/Saale, Germany. marina.fittkau@medizin.uni-halle.de
Purpose:
Mucosal inflammation in the small intestine is a potentially hazardous side effect of abdominal irradiation. In an effort to develop a quantitative method of evaluating mucosal damage, the luminal release of brush border enzymes in response to ionizing radiation was examined using two investigational strategies.
Methods:
First, a 20 cm segment of the proximal jejunum was perfused in situ and enzymatic activities within the perfusates were evaluated. In a second approach, enzymatic activities were directly evaluated in isolated brush border membranes from the jejunal mucosa.
Results:
Most of the peptidase activities measured were increased in the perfusates 1 day after irradiation and had returned to control levels at 4 days. In the brush border membranes, some enzyme activities decreased at 1 day and were, with the exception of leucineaminopeptidase (LAP), similar to control levels at 4 days.
Conclusions:
LAP is more strongly affected by radiation than the transmembranously bounded enzymes.
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