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Related Experiment Video

Updated: May 2, 2026

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

Published on: November 18, 2009

Functional annotation of a full-length mouse cDNA collection.

J Kawai¹, A Shinagawa, K Shibata

¹Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center, Yokohama Institute, Kanagawa, Japan.

|February 24, 2001

Summary

The RIKEN Mouse Gene Encyclopaedia Project sequenced over 21,000 mouse cDNAs, expanding known gene families and discovering new ones. This research provides a comprehensive resource for understanding mouse genome coding potential.

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Last Updated: May 2, 2026

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Published on: November 18, 2009

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An Integrated Approach for Microprotein Identification and Sequence Analysis

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Published on: July 12, 2022

Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

The RIKEN Mouse Gene Encyclopaedia Project aims to comprehensively map the mouse genome.
Understanding the full coding potential requires systematic cDNA sequencing and gene mapping.

Purpose of the Study:

To describe the first RIKEN clone collection, a large set of full-length mouse cDNAs.
To functionally annotate initial cDNA sets through the FANTOM meeting.
To analyze these cDNAs for gene family expansion and novel gene discovery.

Main Methods:

Collection and sequencing of full-length complementary DNAs (cDNAs).
Physical mapping of genes to the mouse genome.
International functional annotation meeting (FANTOM) for cDNA annotation.

Main Results:

Establishment of one of the largest cDNA clone collections for any organism.
Annotation of the first 21,076 cDNAs.
Identification of extended known gene families and novel gene families within the mouse genome.

Conclusions:

The RIKEN clone collection is a valuable resource for mouse genomics.
Analysis of the cDNAs significantly contributes to understanding mouse gene repertoire.
This work advances the systematic determination of the mouse genome's coding potential.