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Functional annotation of a full-length mouse cDNA collection.
J Kawai1, A Shinagawa, K Shibata
1Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center, Yokohama Institute, Kanagawa, Japan.
Nature
|February 24, 2001
Summary
The RIKEN Mouse Gene Encyclopaedia Project sequenced over 21,000 mouse cDNAs, expanding known gene families and discovering new ones. This research provides a comprehensive resource for understanding mouse genome coding potential.
Area of Science:
- Genomics
- Molecular Biology
- Bioinformatics
Background:
- The RIKEN Mouse Gene Encyclopaedia Project aims to comprehensively map the mouse genome.
- Understanding the full coding potential requires systematic cDNA sequencing and gene mapping.
Purpose of the Study:
- To describe the first RIKEN clone collection, a large set of full-length mouse cDNAs.
- To functionally annotate initial cDNA sets through the FANTOM meeting.
- To analyze these cDNAs for gene family expansion and novel gene discovery.
Main Methods:
- Collection and sequencing of full-length complementary DNAs (cDNAs).
- Physical mapping of genes to the mouse genome.
- International functional annotation meeting (FANTOM) for cDNA annotation.
Main Results:
- Establishment of one of the largest cDNA clone collections for any organism.
- Annotation of the first 21,076 cDNAs.
- Identification of extended known gene families and novel gene families within the mouse genome.
Conclusions:
- The RIKEN clone collection is a valuable resource for mouse genomics.
- Analysis of the cDNAs significantly contributes to understanding mouse gene repertoire.
- This work advances the systematic determination of the mouse genome's coding potential.
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