Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A correction to the autoanalyzer data for quantitative agglutination measurements.

C Doinel, C Ropars, C Salmon

    Vox Sanguinis
    |January 1, 1975
    PubMed
    Summary

    This study details a correction factor for the AutoAnalyzer to accurately measure antibody agglutination. This method improves quantitative analysis of Polybrene agglutination assays.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Lung metastases of pancreatic adenocarcinoma: Watch for the second train!]

    Revue des maladies respiratoires·2019
    Same author

    Engineered erythrocytes: influence of P50 rightward shift and oxemia on oxygen transport to tissues.

    Medical & biological engineering & computing·1999
    Same author

    Down-regulation of TSH subunit mRNA levels by thyroid hormones in the European eel.

    General and comparative endocrinology·1997
    Same author

    Internalization and distribution of inositol hexakisphosphate in red blood cells.

    Biotechnology and applied biochemistry·1996
    Same author

    Improved pharmacodynamics of L-asparaginase-loaded in human red blood cells.

    European journal of clinical pharmacology·1996
    Same author

    Tolerance evaluation of L-asparaginase loaded in red blood cells.

    European journal of clinical pharmacology·1996

    Area of Science:

    • Immunology
    • Biochemistry
    • Analytical Chemistry

    Background:

    • Accurate quantification of antibody activity is crucial in immunological assays.
    • The AutoAnalyzer offers automated analysis but can introduce errors in agglutination measurements.
    • The Polybrene agglutination technique is a common method for assessing antibody-mediated cell clumping.

    Purpose of the Study:

    • To address the experimental error in AutoAnalyzer-based antibody agglutination measurements.
    • To introduce and describe a correction factor for quantitative determination of agglutination percentage.
    • To enhance the accuracy of antibody agglutinating activity assessment using the AutoAnalyzer.

    Main Methods:

    • Utilized the AutoAnalyzer for quantitative measurement of antibody agglutinating activity.

    Related Experiment Videos

  • Employed the Polybrene agglutination technique.
  • Developed and described an experimental method for applying a correction factor to account for continuous flow system errors.

    • Main Results:

    • Identified an experimental error inherent in the AutoAnalyzer's continuous flow system for agglutination assays.
    • Established the necessity of a correction factor for accurate determination of the true agglutination percentage.
    • Presented a validated experimental method for applying this correction factor.

    Conclusions:

    • The described correction factor is essential for accurate quantitative measurement of antibody agglutinating activity using the AutoAnalyzer and Polybrene technique.
    • This method improves the reliability of agglutination assays performed on continuous flow systems.
    • The findings contribute to more precise immunological and biochemical analyses.