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Continuous separation of green fluorescent protein by annular chromatography
A Uretschläger1, A Einhauer, A Jungbauer
1Institute of Applied Microbiology, University of Agricultural Sciences, Vienna, Austria.
Journal of Chromatography. A
|February 24, 2001
Abstract:
The concept of annular chromatography was tested by separation of a real protein solution used in biotechnology. Green fluorescent protein was expressed in S. cerevisiae and the extract was continuously separated by a pressurized annular chromatograph packed with a Superdex 200 prep grade size-exclusion chromatography medium. Purity was checked by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blotting and fluorescent intensity. The continuous mode was compared to batchwise operation. Under the assumption that equivalent fractions are collected, both modes are comparable.