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Miniaturized solid-phase extraction with resin disks.

J S Fritz1, J J Masso

  • 1Department of Chemistry and Ames Laboratory, US Department of Energy, Iowa State University, Ames 50011, USA. kniss@ameslab.gov

Journal of Chromatography. A
|February 24, 2001
PubMed
Summary
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Miniaturized solid-phase extraction (SPE) using membrane disks in a 50-microliter syringe enables highly efficient sample preparation. This technique achieves significant concentration factors and excellent recovery rates for trace analytes in aqueous samples.

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Environmental Analysis

Background:

  • Miniaturization is a significant trend in solid-phase extraction (SPE) and chemical analysis.
  • Traditional SPE methods can be time-consuming and require larger sample volumes.
  • Development of smaller-scale extraction techniques is crucial for efficient analysis.

Purpose of the Study:

  • To describe the properties and advantages of membrane disks for miniaturized SPE.
  • To review a semimicro SPE method utilizing small-diameter extraction disks.
  • To evaluate the performance of an ultra-miniaturized SPE device integrated into a syringe.

Main Methods:

  • Utilized membrane disks (4 mm and 0.7 mm diameter) for solid-phase extraction.
  • Employed an ultra-miniaturized SPE device (0.7 mm membrane in a 50-microliter syringe).

Related Experiment Videos

  • Automated sample processing using a single-syringe infusion pump for aqueous samples.
  • Analyzed eight substituted benzenes at 10 ppb concentration levels.

    • Main Results:

    • Achieved 500-fold concentration with 5 microliter elution volumes.
    • Obtained average recoveries greater than 90% with a relative standard deviation (RSD) of 4.6%.
    • Direct gas chromatograph injection of the eluate yielded average recoveries >90% and 6.2% RSD.

    Conclusions:

    • Miniaturized SPE with membrane disks offers significant advantages in sample preparation.
    • The ultra-miniaturized syringe-based SPE system provides high concentration factors and excellent analytical performance.
    • This technique is suitable for trace analysis of organic compounds in aqueous samples with high efficiency and accuracy.