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[The effect of melatonin on enhancing immune function and inhibiting the ability of NO over-release in morphine
1Peking University, Medical Branch, National Key Laboratories of Natural and Biomimetic Drugs, Beijing 100083, China.
Aim:
To observe the effects and mechanism of melatonin (MT) on the immune function of morphine dependent mice.
Methods:
A physical dependent mice model was established by repeated subcutaneous injection of morphine. The intensity of morphine withdrawal syndrome was evaluated according to the weight of immune organs, the proliferation reaction of stimulated splenic lymphocytes by Con A, the phagoindex of blood primed macrophages and the content of NO induced in the peritoneal macrophage (pM phi).
Results:
MT reversed the inhibitory effect of morphine on the proliferation ability of splenic lymphocytes and enhanced the phagocytosis of macrophages of morphine dependent mice obviously and prevented the over-release of NO from pM phi. The enhancing effects of MT on the phagocytosis can be prevented by naloxon.
Conclusion:
MT can significantly enhance the immune function of morphine dependent mice and inhibit NO excessive release from pM phi.