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[Detection of impurities in DNA samples by using the convolution spectrum method]
1Department of Pharmaceutical Analysis, College of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Aim:
To detect the presence of phenol, amino acids and endotoxin in DNA preparations.
Methods:
By using the convolution curve transformation technology, correlation coefficient discriminant which was put forward by spectrum space theory and computer information process technology, impurities in DNA samples were detected.
Results:
The convolution spectrum method compared with A260/A280 ratio method and second-derivative absorption spectrum method could be used for the semiquantitative determinations of impurities in DNA samples and the results were satisfactory.
Conclusion:
The method was simple, convenient, fast and valuable to the biochemical analysis.