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Study of cell-material interaction by estimating NF-kappaB activation in HeLa S3 cells adhered onto hydrophilic
A Kishida1, T Matsuyama, I Kitajima
1Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, Korimoto, Japan. kishida@ri.ncvc.go.jp
Abstract:
In order to interpret how cells recognize biomaterials, nucleic factor-kappa B (NF-kappaB) activation in the attached HeLa S3 cells on various substrates was evaluated. As substrates, materials of hydrophilic nature (cellulose, poly(acrylamide)-grafted poly(ethylene) (PAAm-g-PE), and lipids films) were used. The contemporary assay method for NF-kappaB was modified to fit our system. As a result, NF-kappaB activation varied depending on the substrates. The NF-kappaB outcome was induced significantly in the HeLa S3 cells that had adhered onto the lipid films in a short time. On the other hand, high levels of NF-kappaB induction were observed in the HeLa cells adhered to the celluose and PAAm-g-PE after a 24 h incubation period. The induction of NF-kappaB by cell-material interaction is discussed from the point of view of biocompatibility.