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Related Experiment Videos

Ecological study of a bioaugmentation failure.

T Bouchez1, D Patureau, P Dabert

  • 1Institut National de la Recherche Agronomique, Laboratoire de Biotechnologie de l'Environnement, Narbonne, France.

Environmental Microbiology
|February 28, 2001
PubMed
Summary

Bioaugmentation with Microvirgula aerodenitrificans failed to improve nitrogen removal. Instead, protozoa overgrowth led to nitrification breakdown, highlighting risks of massive bioaugmentation in wastewater treatment.

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Area of Science:

  • Environmental Microbiology
  • Wastewater Treatment Technologies
  • Microbial Ecology

Background:

  • Bioaugmentation is a strategy to enhance microbial processes in wastewater treatment.
  • Nitrification is a crucial step in nitrogen removal, susceptible to microbial community shifts.
  • The aerobic denitrifying bacterium Microvirgula aerodenitrificans was investigated for its potential in enhancing nitrification.

Purpose of the Study:

  • To evaluate the effectiveness of bioaugmentation using Microvirgula aerodenitrificans in a nitrifying sequencing batch reactor.
  • To investigate the impact of bioaugmentation on nitrogen removal efficiency and microbial community dynamics.
  • To understand the ecological interactions influencing the success or failure of bioaugmentation.

Main Methods:

Related Experiment Videos

  • Nitrifying sequencing batch reactor operation with and without bioaugmentation.
  • Fluorescent in situ hybridization (FISH) with rRNA-targeted probes for bacterial identification.
  • Radioactive dot-blot hybridization for monitoring bacterial populations.
  • In situ quantification of probe-targeted bacteria.

    • Main Results:

    • Bioaugmentation with M. aerodenitrificans did not improve nitrogen removal and led to nitrification breakdown in one reactor.
    • Added bacteria rapidly disappeared, with strong signals detected in protozoan digestive vacuoles.
    • Protozoan overgrowth and disappearance of free-living bacteria were observed in the bioaugmented reactor.
    • Nitrifying bacteria populations decreased similarly in both bioaugmented and control reactors.

    Conclusions:

    • Massive bioaugmentation with M. aerodenitrificans can have undesirable effects, including nitrification failure.
    • Predator-prey interactions, specifically protozoan grazing, play a significant role in the fate of introduced microorganisms.
    • Careful consideration of ecological parameters and potential negative impacts is crucial for successful bioaugmentation strategies in wastewater treatment.