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Related Experiment Videos

Ultraweak sugar-sugar interactions for transient cell adhesion.

F Pincet1, T Le Bouar, Y Zhang

  • 1Laboratoire de Physique Statistique de l'Ecole Normale Supérieure, UMR 8550 associée au CNRS et aux Universités Paris 6 et Paris 7, 75231 Paris cedex 05, France.

Biophysical Journal
|February 27, 2001
PubMed
Summary

Specific carbohydrate interactions, like Lewis-x (Le(x)) mediated by calcium, are crucial for cell adhesion. This study quantifies these ultralow forces, explaining their underestimation in biological processes.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Developmental Biology

Background:

  • Carbohydrate-carbohydrate interactions are often overlooked in cell recognition and adhesion.
  • A calcium-mediated interaction between Lewis-x (Le(x)) determinants has been hypothesized to play a role in murine embryogenesis.

Purpose of the Study:

  • To directly quantify the Ca(2+)-mediated homotypic interaction between Lewis-x (Le(x)) determinants.
  • To measure this interaction in a membrane-like environment.

Main Methods:

  • Utilized micropipette aspiration and contact angle measurements.
  • Employed giant vesicles functionalized with Le(x) to mimic cell surfaces.

Main Results:

  • Confirmed the existence of the specific Ca(2+)-mediated Le(x)-Le(x) interaction.

Related Experiment Videos

  • Quantified the ultralow adhesion forces, finding them to be below thermal energy.
  • Demonstrated that significant cell-cell adhesion requires a high concentration of Le(x) molecules.

    • Conclusions:

    • The specific carbohydrate-carbohydrate interaction between Le(x) determinants is confirmed and quantified.
    • The ultralow nature of this interaction explains why it has been historically neglected.
    • These findings highlight the importance of considering specific carbohydrate interactions in cell adhesion and developmental processes.