Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Difference in apoptosis induction between surface IgD and IgM.

D Peckham1, E Andersen-Nissen, F D Finkelman

  • 1Department of Medicine, Iowa City Veterans Administration Medical Center, Iowa City, IA 52242, USA.

International Immunology
|February 27, 2001
PubMed
Summary

Low-dose anti-delta antibodies trigger B cell apoptosis, unlike anti-mu antibodies. This suggests a distinct B cell receptor signaling pathway influencing cell fate and immune tolerance.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ECFS standards of care on CFTR-related disorders: Identification and care of the disorders.

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society·2024
Same author

Assessing the effects of transient weather conditions on airborne transmission risk in naturally ventilated hospitals.

The Journal of hospital infection·2024
Same author

Changes in liver biochemistry and tacrolimus levels following the introduction of elexacaftor/tezacaftor/ivacaftor in patients with cystic fibrosis and liver transplant.

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society·2023
Same author

Intravenous fosfomycin for pulmonary exacerbation of cystic fibrosis: Real life experience of a large adult CF centre.

Pulmonary pharmacology & therapeutics·2018
Same author

Diagnostic and prognostic significance of systemic alkyl quinolones for P. aeruginosa in cystic fibrosis: A longitudinal study; response to comments.

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society·2017
Same author

Detection of drug-responsive B lymphocytes and antidrug IgG in patients with β-lactam hypersensitivity.

Allergy·2016

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • The canonical two-signal model posits B cell activation requires antigen receptor (signal 1) and co-stimulatory signals (signal 2).
  • Signal 1 alone typically induces tolerance or anergy, while signal 1 plus signal 2 promotes proliferation.
  • The differential roles of B cell receptor (BCR) isotypes, like delta and mu, in regulating these outcomes remain incompletely understood.

Purpose of the Study:

  • To investigate the distinct effects of anti-delta versus anti-mu antibody stimulation on resting B cells.
  • To determine if BCR signal 1 alone can induce apoptosis in B cells.
  • To elucidate the molecular events associated with anti-delta-induced B cell apoptosis.

Main Methods:

  • Purified resting B cells from mice were treated with various concentrations of anti-delta or anti-mu antibodies in vitro.

Related Experiment Videos

  • Cell cycle entry (S phase), apoptosis, and expression of co-stimulatory molecules (B7-2, MHC class II) and transcription factors (c-Myc, Egr-1) were assessed.
  • Effects of co-stimulatory signals (anti-CD40, IL-4) on anti-delta-treated B cells were evaluated.

    • Main Results:

    • Low-dose anti-delta (0.1-0.5 µg/ml) induced accelerated apoptosis in B cells without significant cell cycle entry.
    • In contrast, anti-mu antibodies did not induce early apoptosis; higher doses of anti-delta (50 µg/ml) caused modest proliferation.
    • Anti-delta treatment upregulated B7-2 and MHC class II on apoptotic B cells and induced transient c-Myc/Egr-1 expression, a pattern linked to apoptosis.

    Conclusions:

    • Cross-linking the delta BCR, but not the mu BCR, with low-dose antigen receptor stimulation can lead to B cell apoptosis.
    • A B cell receptor stimulus sufficient for early activation events but insufficient for full cell cycle entry may trigger programmed cell death.
    • These findings reveal a functional divergence between delta and mu BCR signaling in resting B cells, impacting immune tolerance.