Related Experiment Video
Updated: Jun 21, 2026

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry
Published on: May 22, 2012
Requirement of a centrosomal activity for cell cycle progression through G1 into S phase
E H Hinchcliffe1, F J Miller, M Cham
1Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA., Laboratory of Cell Regulation, Wadsworth Center, Albany, NY 12201, USA.
Abstract:
Centrosomes were microsurgically removed from BSC-1 African green monkey kidney cells before the completion of S phase. Karyoplasts (acentrosomal cells) entered and completed mitosis. However, postmitotic karyoplasts arrested before S phase, whereas adjacent control cells divided repeatedly. Postmitotic karyoplasts assembled a microtubule-organizing center containing gamma-tubulin and pericentrin, but did not regenerate centrioles. These observations reveal the existence of an activity associated with core centrosomal structures-distinct from elements of the microtubule-organizing center-that is required for the somatic cell cycle to progress through G1 into S phase. Once the cell is in S phase, these core structures are not needed for the G2-M phase transition.
More Related Videos
Related Concept Videos
Interphase
The Cell Cycle Control System
Cells Coordinate Growth and Proliferation
The Cell Cycle Control System
Cells Coordinate Growth and Proliferation
The Cell Cycle Control System
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...

