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The two human chymotrypsinogens. Purification and characterization.

A De Caro, C Figarella, O Guy

    Biochimica Et Biophysica Acta
    |February 27, 1975
    PubMed
    Summary
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    Human pancreatic juice contains two distinct chymotrypsinogens: Chymotrypsinogen A (major, acid-stable) and Chymotrypsinogen B (minor, acid-unstable). Both were purified and characterized, revealing differences in molecular weight and activity.

    Area of Science:

    • Biochemistry
    • Proteomics
    • Human Physiology

    Background:

    • Human pancreatic juice contains precursor forms of digestive enzymes called zymogens.
    • Chymotrypsinogen is a key zymogen involved in protein digestion.
    • Understanding human pancreatic zymogens is crucial for digestive health research.

    Purpose of the Study:

    • To purify and characterize the two distinct chymotrypsinogen forms found in human pancreatic juice.
    • To compare the biochemical properties of human chymotrypsinogen A and B.
    • To identify and partially characterize other enzymatic components in pancreatic juice.

    Main Methods:

    • Purification of chymotrypsinogens from human pancreatic juice.
    • Electrophoretic analysis to assess protein differences.

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  • Immunological assays to determine distinctness.
  • Enzymatic activity assays (N-acetyl-L-tyrosine ethylester).
  • Molecular weight determination.
  • Amino acid composition analysis.

    • Main Results:

    • Two immunologically and electrophoretically distinct chymotrypsinogens were purified: Chymotrypsinogen A and Chymotrypsinogen B.
    • Chymotrypsinogen A is the major component (90% activity), acid-stable, with a molecular weight of ~24,000 and specific activity of 530.
    • Chymotrypsinogen B is a minor component (7% activity), acid-unstable, with a molecular weight of ~27,000 and specific activity of 300.
    • A minor proelastase component (~3% activity) was also partially characterized.

    Conclusions:

    • Human pancreatic juice contains at least two distinct chymotrypsinogen forms with differing biochemical properties.
    • Chymotrypsinogen A is the predominant, acid-stable form, similar to bovine and porcine counterparts.
    • Chymotrypsinogen B is a less abundant, acid-labile form, presenting unique characteristics.