Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Aedes aegypti peroxidase gene characterization and developmental expression.

X Zhao¹, C T Smartt, J Li

¹Department of Animal Health and Biomedical Sciences, 1656 Linden Dr., University of Wisconsin, Madison, WI 53706, USA.

Insect Biochemistry and Molecular Biology

|February 27, 2001

Summary

Researchers isolated the Aedes aegypti peroxidase (AePox) gene, revealing its structural similarity to human myeloperoxidases. This mosquito peroxidase is expressed in larvae and pupae, suggesting a role in insect development and immunity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Development and fundamental psychometric properties of the Magnetic Resonance Imaging Child-Anxiety Questionnaire (MRIC-AQ).

Radiography (London, England : 1995)·2025

Same author

Molecular surveillance detects high prevalence of the neglected parasite <i>Mansonella ozzardi</i> in the Colombian Amazon.

medRxiv : the preprint server for health sciences·2023

Same author

Hemocyte classification of three mosquito vectors: Aedes togoi, Anopheles lesteri and Culex quinquefasciatus.

Tropical biomedicine·2021

Same author

Surgical repair of parastomal bulging: a retrospective register-based study on prospectively collected data.

Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2020

Same author

Widespread evidence for interspecific mating between Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in nature.

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases·2015

Same author

The dual roles of Armigeres subalbatus prophenoloxidase V in parasite melanization and egg chorion melanization in the mosquito Ar. subalbatus.

Insect biochemistry and molecular biology·2015

Area of Science:

Insect molecular biology
Biochemistry
Genomics

Background:

Insect peroxidases perform vital functions including detoxification, extracellular matrix stabilization, and immune responses.
The specific roles and characteristics of peroxidases in mosquitoes remain incompletely understood.

Purpose of the Study:

To isolate and characterize the peroxidase gene (AePox) from the mosquito Aedes aegypti.
To investigate the structural, functional, and expression patterns of AePox.

Main Methods:

Gene isolation and cDNA sequencing of AePox from Aedes aegypti.
Bioinformatic analysis for protein homology and domain identification.
Gene expression studies using various mosquito life stages and tissues.

Related Experiment Videos

Main Results:

AePox encodes a protein homologous to heme-peroxidases, with highest identity to Drosophila melanogaster peroxidase.
Conserved domains suggest a similar structure and catalytic mechanism to mammalian myeloperoxidases.
AePox is transcribed in larvae and pupae but not in adults, ovaries, or early embryos, yet the protein is present across all stages.

Conclusions:

AePox shares structural and potential functional similarities with mammalian myeloperoxidases.
The distinct expression pattern suggests AePox plays a role in specific mosquito developmental stages.
AePox regulation may involve unique elements like ecdysone response elements (EcRE).