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Enhanced green fluorescent protein as a useful tag for rapid identification of homozygous transgenic mice
1Molecular Medicine Research Center, The Institute of Medical Sciences, Tokai University, Bohseidai, Isehara, 259-1193, Kanagawa, Japan. masasato@is.icc.u-tokai.ac.jp
Biomolecular Engineering
|February 27, 2001
Abstract:
We developed a technique that simplifies the process of confirming homozygous transgenics at preimplantation stages, which are the earliest stages used in test breeding, using enhanced green fluorescent protein as a tag. All the blastocysts obtained by mating with the combination of Tg/Tg male (homozygous for transgene) x +/+ female exhibited fluorescence.