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Related Experiment Videos

Bispecific human IgG by design.

P Carter1

  • 1Department of Molecular Oncology, Genentech Inc, 1 DNA Way, South San Francisco, CA 94080-4990, USA. carterp@immunex.com

Journal of Immunological Methods
|February 27, 2001
PubMed
Summary
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Developing bispecific antibodies for therapy faced production challenges. A new technology enables efficient production of bispecific IgG antibodies, overcoming previous limitations and paving the way for clinical development.

Area of Science:

  • Biotechnology
  • Immunology
  • Protein Engineering

Background:

  • Bispecific antibodies offer therapeutic potential but face manufacturing hurdles.
  • Previous methods for bispecific antibody fragments (e.g., diabodies) from E. coli are efficient.
  • Coexpression of bispecific IgG antibodies is inefficient due to chain mispairing.

Purpose of the Study:

  • To review a novel technology for producing bispecific IgG antibodies.
  • To highlight advancements overcoming production challenges for complex antibody therapeutics.

Main Methods:

  • Review of a robust technology for bispecific IgG creation.
  • Focus on methods that minimize IgG contaminants.

Main Results:

Related Experiment Videos

  • The reviewed technology effectively precludes IgG contaminants in bispecific antibody production.
  • Efficient production of bispecific IgG antibodies is now achievable.

    • Conclusions:

    • This technology is expected to accelerate clinical development of bispecific IgG antibodies.
    • The advancement will also benefit other bifunctional Fc-containing molecules, including antibody/immunoadhesin hybrids.