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Updated: Aug 9, 2026
Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
Published on: November 1, 2012
Crystallization of the Bacillus subtilis RTP-DNA complex prepared using NMR spectroscopy
J P Vivian1, J A Wilce, A F Hastings
1Department of Pharmacology/Crystallography Centre, University of Western Australia and the Western Australian Institute for Medical Research, Nedlands, WA 6907, Australia.
Abstract:
The replication terminator protein (RTP)-DNA complex of Bacillus subtilis is responsible for the arrest of DNA replication at terminator sites in the B. subtilis chromosome. The crystallization and preliminary diffraction data analysis for the complex of an (15)N-labelled mutant form of RTP and a symmetrical form of its DNA-binding site is reported. NMR spectroscopy was used to assess the stoichiometry of complex formation, with the sample containing the most homogeneous solution of complex giving rise to diffracting crystals. Synchrotron-radiation data to 2.5 A were collected from a crystal of space group P3(2)21, unit-cell parameters a = b = 44.780, c = 395.582 A, containing an RTP dimer within the asymmetric unit.
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