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Purification, crystallization and preliminary crystallographic analysis of the periplasmic binding protein ProX from
1Fachbereich Biologie, Universität Konstanz, 78457 Konstanz, Germany. jason.breed@astrazeneca.com
Acta Crystallographica. Section D, Biological Crystallography
|February 27, 2001
Abstract:
A periplasmic binding protein (ProX) for the compatible solutes glycine betaine and proline betaine from Escherichia coli was crystallized using the hanging-drop vapour-diffusion method. Crystals were grown using a protein concentration of 10 mg ml(-1) and a precipitant of 26-28% PEG 4000 in 50 mM PIPES pH 6.2-6.4. Native diffraction data to 1.93 A resolution have been obtained from crystals at 290 K. The crystals belong to the space group P2(1)2(1)2(1), with unit-cell parameters a = 48.1, b = 55.0, c = 115.7 A, and contain one molecule per asymmetric unit.