Social interaction with an intoxicated sibling can result in increased intake of ethanol by periadolescent rats

A novel procedure for enhancing voluntary intake of ethanol in periadolescent rats is described. The procedure is a modification of Galef et al.'s (e.g., Galef, Kennett, & Stein, 1985; Anim Learn Behave 13:25-30) demonstrator-observer procedure. Subjects were Sprague-Dawley rats, 28-35 days of age. The experimental subject (observer) interacted with a same-sex conspecific (demonstrator) previously administered (a) 1.5 g/kg ethanol, (b) an equal volume of water, or (c) 2.1% Sanka coffee intragastrically. Observers were tested with 24-hour access to ethanol and coffee solutions. Observers that had interacted with demonstrators administered ethanol ingested significantly more ethanol during the test than observers in the other two groups. In Experiment 2 demonstrators were administered one of several doses of ethanol (0.0, 1.0, 1.5, or 3.0 g/kg) and observers' ethanol intakes were assessed. Only those observers that interacted with 1.5 g/kg demonstrators increased their ingestion of ethanol, relative to water controls. The lower (1.0 g/kg) and higher (3.0 g/kg) dose groups did not show altered ethanol ingestion. These results are discussed with respect to threshold levels of respired ethanol cues and the ability of observers to detect these cues from demonstrators. The demonstrator-observer procedure appears to be effective for the social transmission of preferences for ethanol in periadolescent rats.