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YJL159w does encode Pir2/Hsp150
1Sección Departamental de Microbiología, Facultad de Farmacia, Universidad de Valencia, Avda. Vicente Andres Estelles s/n, 46100-Burjassot, Valencia, Spain.
Abstract:
In this paper we compare the sequence of the gene HSP150/PIR2, independently determined by two different groups, with that present in the yeast database as YJL159w, determined within the Yeast Sequencing Project. Although YJL159w is believed to encode Hsp150/Pir2, there are important differences between the amino acid sequence coded by this ORF and that of HSP150/PIR2. To find out if this divergence is due to strain polymorphism or to a possible sequencing error, we have analysed the diverging zone of this ORF in three strains and have found it entirely consistent with the sequence reported as HSP150/PIR2, concluding that the divergence is probably due to a sequencing error in YJL159w.
Insights
Comparing the HSP150/PIR2 gene sequence with the yeast database entry YJL159w revealed significant differences. Analysis confirmed these variations are likely due to a sequencing error in YJL159w, not strain polymorphism.
Area of Science:
- Molecular Biology
- Yeast Genetics
- Bioinformatics
Background:
- The gene HSP150/PIR2 is crucial in yeast.
- The Yeast Sequencing Project identified a related open reading frame (ORF) as YJL159w.
- Previous studies suggested YJL159w encodes Hsp150/Pir2.
Purpose of the Study:
- To resolve discrepancies between the independently determined HSP150/PIR2 gene sequence and the YJL159w database entry.
- To investigate whether observed sequence differences are due to strain polymorphism or potential sequencing errors.
Main Methods:
- Comparative sequence analysis of HSP150/PIR2 and YJL159w.
- PCR amplification and sequencing of the divergent region of the YJL159w ORF in three different yeast strains.
Main Results:
- Significant amino acid sequence differences were identified between the reported HSP150/PIR2 and the YJL159w sequences.
- Sequence analysis of the divergent zone in three yeast strains confirmed the HSP150/PIR2 sequence.
- The analyzed region in the tested strains was consistent with the HSP150/PIR2 sequence, not YJL159w.
Conclusions:
- The observed divergence between HSP150/PIR2 and YJL159w is unlikely to be caused by strain polymorphism.
- Evidence strongly suggests a sequencing error in the YJL159w entry within the yeast database.