Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The membrane current of single rod outer segments.

D A Baylor, T D Lamb, K W Yau

    The Journal of Physiology
    |March 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Fine needle biopsy guided by contrast-enhanced harmonic versus conventional endoscopic ultrasound with macroscopic on-site evaluation for solid pancreatic lesions: abridged secondary publication.

    Hong Kong medical journal = Xianggang yi xue za zhi·2026
    Same author

    When qualitative data contradict quantitative data: diabetes distress in the Chinese-Canadian community.

    Diabetic medicine : a journal of the British Diabetic Association·2019
    Same author

    Emergency femoral hernia repair: 13-year retrospective comparison of the three classical open surgical approaches.

    Hernia : the journal of hernias and abdominal wall surgery·2016
    Same author

    Why rods and cones?

    Eye (London, England)·2015
    Same author

    Surgical morbidity and mortality in obturator hernia: a 10-year retrospective risk factor evaluation.

    Hernia : the journal of hernias and abdominal wall surgery·2013
    Same author

    Ectopic expression of cone-specific G-protein-coupled receptor kinase GRK7 in zebrafish rods leads to lower photosensitivity and altered responses.

    The Journal of physiology·2011

    Individual toad retinal rod outer segments generate outward currents in response to light flashes. Light intensity affects response amplitude, and background light modulates sensitivity and kinetics, revealing insights into visual transduction mechanisms.

    Area of Science:

    • * Visual Neuroscience
    • * Phototransduction
    • * Retinal Physiology

    Background:

    • * Understanding the cellular mechanisms of light detection in the retina is crucial for comprehending visual processing.
    • * Previous studies using intracellular voltage recordings suggested complex responses to light, including a relaxation phase.

    Purpose of the Study:

    • * To directly record membrane currents from individual rod outer segments to elucidate the biophysical basis of phototransduction.
    • * To investigate the relationship between light intensity and response amplitude.
    • * To characterize the kinetics and spectral sensitivity of toad rods.

    Main Methods:

    • * Isolation and suction pipette recording of membrane currents from individual toad (Bufo marinus) rod outer segments.

    Related Experiment Videos

  • * Application of controlled light flashes of varying intensity and spectral composition.
  • * Local and diffuse illumination of the recorded outer segment.

    • Main Results:

    • * Light flashes evoked transient outward currents, saturating at approximately 1 photon/µm².
    • * Response amplitude scaled linearly with outer segment length, suggesting uniform channel density.
    • * Responses lacked the previously observed relaxation, indicating it does not originate in the outer segment; spectral sensitivity peaked at 498 nm.

    Conclusions:

    • * The study provides direct evidence for light-evoked conductance changes in the rod outer segment.
    • * Findings suggest a multi-step transduction process with delays and highlight the role of background light in adapting visual sensitivity.
    • * The data supports a model of phototransduction involving a uniform distribution of light-sensitive channels along the outer segment.