Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

PLC-beta1, activated via mGluRs, mediates activity-dependent differentiation in cerebral cortex.

A J Hannan1, C Blakemore, A Katsnelson

  • 1University Laboratory of Physiology, Parks Road, Oxford OX1 3PT, UK.

Nature Neuroscience
|February 27, 2001
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Attenuated responses to attention-modulating drugs in the neuroligin-3<sup>R451C</sup> mouse model of autism.

Journal of neurochemistry·2024
Same author

Chronically high stress hormone levels dysregulate sperm long noncoding RNAs and their embryonic microinjection alters development and affective behaviours.

Molecular psychiatry·2023
Same author

Assessing attention orienting in mice: a novel touchscreen adaptation of the Posner-style cueing task.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology·2020
Same author

Limitations to intergenerational inheritance: subchronic paternal stress preconception does not influence offspring anxiety.

Scientific reports·2020
Same author

Environmental enrichment modulates affiliative and aggressive social behaviour in the neuroligin-3 R451C mouse model of autism spectrum disorder.

Pharmacology, biochemistry, and behavior·2020
Same author

Isoform specific differences in phospholipase C beta 1 expression in the prefrontal cortex in schizophrenia and suicide.

NPJ schizophrenia·2017

Glutamate signaling via phospholipase C-beta1 (PLC-beta1) and metabotropic glutamate receptors (mGluRs) is crucial for coordinated neocortical development. Disrupting this pathway impairs the differentiation of sensory processing areas in the mouse brain.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Developmental Biology

Background:

  • Neocortical development involves coordinated axonal pathfinding and neuronal differentiation.
  • Glutamate neurotransmission plays a key role in orchestrating these developmental processes.

Purpose of the Study:

  • To investigate the role of phospholipase C-beta1 (PLC-beta1) and metabotropic glutamate receptors (mGluRs) in neocortical development.
  • To determine if glutamate signaling is essential for the formation of functional cortical circuits.

Main Methods:

  • Utilized knockout mouse models lacking PLC-beta1 or mGluR5.
  • Examined cytoarchitectural differentiation of 'barrels' in the somatosensory cortex.
  • Assessed thalamic innervation patterns and phosphoinositide hydrolysis.

Related Experiment Videos

Main Results:

  • Homozygous null mutations in PLC-beta1 or mGluR5 disrupted barrel cytoarchitecture.
  • Thalamic innervation patterns remained largely segregated in mutant mice.
  • Group 1 mGluR-stimulated phosphoinositide hydrolysis was significantly reduced in PLC-beta1 knockout mice.

Conclusions:

  • PLC-beta1 activation by mGluR5 is critical for coordinated neocortical development.
  • Presynaptic and postsynaptic contributions to cortical differentiation can be genetically separated.
  • This signaling pathway is essential for the precise formation of sensory maps.