Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Development and characterization of tissue-engineered aortic valves.

J Zeltinger1, L K Landeen, H G Alexander

  • 1Advanced Tissue Sciences, Inc., La Jolla, California 92037, USA.

Tissue Engineering
|February 27, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Causes of loss to follow-up from drug-resistant TB treatment in Khayelitsha, South Africa.

Public health action·2022
Same author

Leveraging nurse practitioner capacities to achieve global health for all: COVID-19 and beyond.

International nursing review·2020
Same author

Rat bone-marrow cell proliferation and differentiation as an index of the effects of xenobiotics in vitro.

Toxicology in vitro : an international journal published in association with BIBRA·2010
Same author

Effect of pore size and void fraction on cellular adhesion, proliferation, and matrix deposition.

Tissue engineering·2001
Same author

Scaffold-based three-dimensional human fibroblast culture provides a structural matrix that supports angiogenesis in infarcted heart tissue.

Circulation·2001
Same author

A model system for long-term serum-free suspension organ culture of human fetal tissues: experiments on digits and skin from multiple body regions.

Cell and tissue research·1997

Tissue-engineered heart valves were created using human cells on decellularized pig valves. These recellularized valves show cell viability, migration, and matrix production, offering potential benefits over current treatments.

Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Current medical therapies for aortic valve disease have limitations.
  • Tissue engineering offers a promising alternative for creating functional heart valves.
  • Decellularized biological scaffolds provide a foundation for recellularization.

Purpose of the Study:

  • To develop tissue-engineered aortic valves using human neonatal fibroblasts and decellularized porcine aortic valves.
  • To evaluate the viability, migration, and extracellular matrix (ECM) synthesis of human fibroblasts within the scaffold.
  • To assess the potential of a novel bioreactor system in promoting cell colonization under dynamic conditions.

Main Methods:

  • Human neonatal fibroblasts were seeded onto decellularized porcine aortic valve scaffolds.

Related Experiment Videos

  • Cultures were maintained for up to 8 weeks in a novel bioreactor with dynamic pulsatile flow.
  • Cell viability was assessed using MTT staining.
  • Cellular infiltration and colonization were evaluated using Hematoxylin & Eosin staining and immunocytochemistry for prolyl-4-hydroxylase.
  • Extracellular matrix synthesis was quantified using radiolabeled thymidine and proline analog studies.

    • Main Results:

    • Human fibroblasts successfully attached to and migrated into the decellularized porcine valve scaffold under both static and dynamic flow conditions.
    • MTT viability staining confirmed the survival of human cells within the scaffold.
    • Histological and immunocytochemical analyses demonstrated gradual colonization and integration of human cells.
    • Fibroblasts exhibited mitotic activity and synthesized human extracellular matrix proteins, supplementing the native porcine matrix.
    • The novel bioreactor facilitated cell colonization under dynamic pulsatile flow.

    Conclusions:

    • This study successfully generated tissue-engineered aortic valves populated with viable human cells.
    • The approach demonstrates the potential for human fibroblasts to colonize and remodel decellularized porcine scaffolds.
    • The use of a dynamic bioreactor supports cell viability and matrix production.
    • This method presents a promising strategy for developing functional heart valves with potential advantages over existing therapies.